范六民 |
科研领域描述
本实验室主要兴趣在于：
1 赤霉素信号与逆境及脱落酸信号之间的交互作用。
赤霉素合成或信号转导中某些关键基因的突变导致作物矮化，可增强作物的抗逆能力并提高作物的产量，故被称为绿色革命基因。对赤霉素信号转导分子机理的解析既具有重要的理论意义又有广阔的应用前景。我们以拟南芥和水稻作为模式植物，通过基因组学、分子遗传学、生物化学等方法和手段对植物发育过程中赤霉素信号转导过程进行深入和系统的研究，以期发现赤霉素信号转导过程中的新的组成分子，研究它们与已知蛋白之间交互作用的分子网络。
逆境是影响植物生长发育的主要环境因子。逆境信号转导中脱落酸起非常重要的作用。我们以拟南芥和水稻作为模式植物进行逆境及脱落酸信号转导途径中关键因子的发掘，研究它们的生物学功能及其与农艺形状的关系。另外一个研究重点是，用遗传学、生物化学和分子遗传学方法揭示植物发育及逆境中赤霉素与脱落酸信号之间的交互作用。
Research Description:
The research interests are mainly focused on understanding the mechanisms underlying plant stress and phytohormone gibberellin signal transduction by taking advantages of multiple approaches involving functional genomics, molecular genetics, biochemical and other approaches, aiming to find novel components in stress and gibberellin signaling pathways, and we are also interested in exploring epigenetic regulatory machinery for gibberellin and stress-responsive gene expression. Another interest is to investigate the cross-talk between gibberellin and stress hormone abscisic acid in plant development and environmental responses.
代表性论文
1. Gao F, Zhou Y, Zhu W, Li X, Fan LM, Zhang G , Proteomic analysis of cold stress-responsive proteins , Planta , 2009 , 230:1033–1046
2. Qiao W, Xiao S, Yu L, Fan LM (corresponding author) , . (2009) Expression of a rice gene OsNOA1 re-establishes nitric oxide synthesis and stress-related gene expression for salt tolerance in Arabidopsis nitric oxide-associated 1 mutant Atnoa1 , Environmental and Experimental Botany , 2009 , 65:90-98.
3. Wang F, Zhu D, Huang X, Li S, Gong Y, Yao Q, Fu X, Fan LM, Deng XW , Biochemical Insights on Degradation of Arabidopsis DELLA Proteins Gained From a Cell-Free Assay System , Plant Cell , 2009 , Aug 28
4. Zhang W, Fan LM (corresponding author). , Actin dynamics regulates voltage-dependent calcium-permeable channels of the Vicia faba guard cell plasma membrane , J Integr Plant Biol , 2009 , 51:912-921.
5. Fan LM, Zhang W, Chen JG, Taylor JP, Jones AM, Assmann SM , Abscisic acid regulation of guard-cell K+ and anion channels in Gβ and RGS–deficient Arabidopsis lines , PNAS , 2008 , 105: 8476-8481.
6. Qiao W, Fan LM (corresponding author) , Nitric oxide signaling in plant responses to abiotic stresses. , Journal of Integrative Plant Biology , 2008 , 50: 1238–1246
7. Zhang W, Fan LM, Wu WH , Osmo-sensitive stretch-activated calcium channels in Vicia faba guard cells are regulated by actin dynamics , Plant Physiol , 2007 , 143(3):1140-51
8. Bai SL, Zhong X, Ma L, Zheng W, Fan LM, Wei N, Deng XW , A simple and reliable assay for detecting specific nucleotide sequence variations in plants using optical thin-film biosensor chips , Plant Journal , 2007 , 49(2): 354-66
9. Ding L, Fan LM, Assmann SM , pgd1, an Arabidopsis thaliana deletion mutant, is defective in pollen germination , Sex Plant Reprod Online , 2007 , 53: 248-263.
10. Fan LM* (corresponding author), Feng X, Wang Y, Deng XW* (corresponding author). , Gibberellin signal transduction in rice , J Integr Plant Biol , 2007 , 49: 731-741. , Review
11. Feng S, Martinez C, Gusmaroli G, Wang Y, Zhou J, Wang F, Chen L, Yu L, Iglesias-Pedraz JM, Kircher S, Schafer E, Fu X, Fan L-M, Deng XW , Coordinated regulation of Arabidopsis development by light and plant hormone gibberellins , Nature , 2007 , 451: 475-479.
12. Zhou J, Wang X, Jiao Y, Qin Y, Liu X, He K, Chen C, Ma L, Wang J, Xiong L, Zhang Q, Fan LM, Deng XW , Rice employs largely organ-specific programs in regulation of genome expression in response to drought and high-salinity stresses , Plant Mol Biol , 2007 , 63: 591-608.
13. Fan LM, Zhao ZX, Assmann SM , Guard cells: a dynamic signaling model , Curr Opin Plant Biol , 2004 , 7: 537-546. , Review
14. Wang YF, Fan LM, Zhang WZ, Zhang W, Wu WH , Ca2+-permeable channels in the plasma membrane of Arabidopsis pollen protoplasts are regulated by actin microfilaments , Plant Physiol , 2004 , 136: 3892-3904
15. Coursol S*, Fan LM* (* contributed equally), Le Stunff H, Gilroy S, Speigel S, Assmann SM , Sphingolipid signalling in guard cells involves G proteins , Nature , 2003 , 423:651-654.
16. Fan LM, Wang YF, Wang H, Wu WH , Outward K+ channels in Brassica chinensis pollen protoplasts are regulated by external and internal pH , Protoplasma , 2003 , 220:143-152.
17. Fan LM, Wang YF, Wang H, Wu WH , In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts , J Exp Bot , 2001 , 52: 1603-1614
18. Fan LM, Wu WH , External pH regulates the inward K+ channels in the plasma membranes of Brassica pollen protoplasts , Prog Nat Sci , 2000 , 10: 68-73.
19. Fan LM, Wu WH, Yang HY , Identification and characterization of the K+ inward currents in the plasma membranes of Brassica pollen protoplasts , Plant Cell Physiol , 1999 , 40: 859-865. |
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