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郭红卫

主要成员:
安丰英,魏华丽,唐中华,李中海,何文容,李文阳,张新岩,李明喆,万雯,俞强,施慧,温兴,冯莹,纪玉锶,薛昶,张兴,朱颖,马梦迪,郝冬冬,邢秋晨,刘婷,郭棣,李聪,李佩涵
Laboratory members:
Fengying An, Huali Wei,Zhonghua Tang,Zhonghai Li, Wenrong He,Wenyang Li,  Xinyan Zhang,  Mingzhe Li, Qiang Yu, Wen Wan, Hui Shi, Xin Wen,Feng Ying,Yusi Ji, Chang Xue, Xing Zhang,Ying Zhu,Mengdi Ma,Dongdong Hao,Qiuchen Xing, Ting Liu,Di Guo,Cong Li,Peihan Li

主要研究方向:
1.气体激素乙烯的信号转导及植物激素和环境信号相互作用的分子机制
2.植物器官衰老的激素调控机理
3.小RNA分子和表观遗传修饰在激素作用中的调控机理
4.植物激素作用途径的演化和系统生物学研究

Research Directions:
1. Signaling mechanisms of plant gaseous hormone ethylene,and interplays among plant hormones and environmental signals in Arabidopsis
2. Hormonal regulation of plant ageing
3. Small RNAs and epigenetic control in plant hormone actions
4. Evolutionary and systems biology studies of plant hormones

Summary of research interests:
As sessile organisms, plants are capable to respond to those internal and external signals throughout their life cycle. Plants integrate endogenous and outward information and employ signal transduction process to survive and thrive in their surrounding environments. During the long course of evolution, plants obtain a high level of growth plasticity by adopting numerous strategies and complex signaling networks. Essential to this plant-environment interaction are the actions of a number of small molecules called plant hormones, including auxins, cytokinins, gibberellins, ethylene, abscisic acid, brassinosteroids, jasmonates and salicylic acid.

Plant hormones play a critical role in growth, development, defense and adaptation responses to environment. A fundamental question in plant biology is how plants respond to various plant hormones at the molecular, physiological, and morphological levels. An equally important question is how those plant hormones act coordinately (in parallel, antagonistically or cooperatively) to regulate plant growth, development and defense responses. To address these questions, we use molecular genetics, biochemistry, genomics, bioinformatics, systems biology and chemical biology approaches to investigate the signaling mechanisms and regulatory networks of plant hormones, particularly the gaseous hormone, ethylene.

I: How do plants sense and respond to ethylene?
Ethylene is a simple gaseous hormone in plants. It plays important roles in plant growth, development and stress tolerance. To date, more than a dozen ethylene response mutants have been identified and the majority of the corresponding genes in these mutants have been cloned. Although the genetic composition of the ethylene signaling pathway is becoming clear, the biochemical features and regulatory mechanisms of each step, or each component, are still poorly understood. Currently we are focusing on the events of transcriptional regulation and protein degradation in the ethylene signal transduction pathway.

II: How does ethylene cross-talk with other signals?
Ethylene has been shown to interact with many signals including JA, auxin, ABA, SA, GA, cytokinins, as well as environmental signals including light, temperature, and nutrients. Those interactions imply the existence of extensive cross-talks and complicated signaling networks in the ethylene regulation of plant growth, development and defense responses. Currently, we are investigating the molecular and biochemical mechanisms of hormones interplays, and identifying the key integration components and central nodes in the signaling networks.

III: Epigenetic control of plant hormone signaling
Small RNA was recently found to be a new growth regulator in plant hormone signaling. We have found that XRN4/EIN5, a ribonuclease, can degrade selected mRNAs including the 3’ products of EBF1/2, which encode two F-box proteins responsible for EIN3/EIL1 destruction. Recent studies further demonstrated that the XRN4/EIN5 exonuclease is a key component of gene silencing pathway, implying an epigenetic regulation in ethylene signaling. Currently we are researching the role and action mode of small RNAs, particularly the 3’UTR fragments, and gene silencing machinery in the hormone perception.

IV: How were ethylene and its signaling pathway evolved?
A very interesting question is when and why a given plant hormone (e.g. ethylene) was adopted by plants as a hormone? And how was its perception and signaling pathway evolved during the evolution course? Currently we are using bioinformatics, comparative genomics, and more systems biology approaches to identify and analyze the orthologue genes of components involved in ethylene responses in several plant species. We are particularly interested in the ethylene responses and its action modes in the moss plant, Physcomitrella patens.


V: Construction and modeling of plant hormones networks
It has been found that many biological processes are coordinately regulated by multiple hormones. We have built an Arabidopsis Hormone Database (AHD: http://ahd.cbi.pku.edu.cn/) by integrating a large body of experimental data and computational annotations. The aim of the Arabidopsis hormone database is to provide a systematic and comprehensive view of morphological phenotypes and regulatory genes participating in numerous plant hormone responses. In the future, we will generate an atlas of plant hormone biology, and make theoretical models to describe, verify and predict the functions and actions of plant hormones.

VI: Hormonal regulation of plant ageing
Plant ageing is quite distinctive and unique compared to animal or human ageing. The proper control of plant ageing programs (such as leaf senescence, flower senescence) has tremendous potential for agricultural good and thus is of great interest to scientists in recent years. The leaf senescence is the final developmental phase of a plant leaf, which is influenced by several phytohormones, with cytokinin and ethylene having the most important roles in delaying or inducing leaf senescence, respectively. Currently we are interested in uncovering the regulatory mechanisms of ethylene and cytokinin on leaf senescence. Also, we have recently built a Leaf Senescence Database (LSD: http://www.eplantsenescence.org/), and attempt to study leaf senescence in a more systematic way.
 
主要发表文章:
1.Wen X.,Zhang C.,Ji Y.,Zhao Q.,He W.,An F.,Jiang L.,Guo H.(2012).Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus.Cell Res.2012.Oct 16

2.Zhong S.,Shi H.,Xue C.,Wang L.,Xi Y.,Li J.,Quail PH.,Deng XW.,Guo H.(2012).Amolecular framework of light-conrolled phytohormone action in Arabidopsi.Curr Biol.2012 Aug 21;22(16):1530-5.

3.An F., Zhang X., Zhu Z., Ji Y., He W., Jiang Z., Li M., Guo H.(2011). Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings.Cell Res.22(5):915-27.

4.Guo H.(2011). Understanding the mode of phytohormones' action in plants.Sci China Life Sci.54(11):1062-3.

5.Stepanova AN, Yun J., Robles LM., Novak O., He W., Guo H., Ljung K., Alonso JM.(2011). The Arabidopsis YUCCA1 flavin monooxygenase functions in the indole-3-pyruvic acid branch of auxin biosynthesis.Plant Cell.23(11):3961-73.

6. He, W., Brumos J., Li, H., Ji, Y., Ke, M., Gong, X., Zeng, Q., Li, W., Zhang, X., An, F., Wen, X., Li, P., Chu, J., Sun, X., Yan, C., Yan N., Xie, D., Raikhel, N., Yang, Z., Stepanova, A.N., Alonso, J.M. and Guo, H. , A small-molecule screen identifies L-Kynurenine as a competitive inhibitor of TAA1 in ethylene-directed auxin biosynthesis and root growth. , The Plant Cell. , 2011 Nov;23(11):3944-60.

7. Zhu, Z., An, F., Feng, Y., Li, P., Xue, L., A, M., Jiang, Z., Kim, J., To, T., Li, W., Yu, Q., Dong, Z., Chen, W., Seki, M., Zhou, J. and Guo, H. , Release of JAZ- and HDA6-associated repression on EIN3/EIL1 mediates jasmonate and ethylene signaling synergy in Arabidopsis. , Proc. Natl. Acad. Sci. USA. , 2011 , 108:12539-44.

8. Liu X., Li Z. Jiang Z., ZhaoY., Peng J., Jin J., Guo H*., Luo J. , LSD: a leaf senescence database. , Nucleic Acids Research. , 2011 , 39: D1103–D1107. ( *Co-Corresponding Author)

9. Jiang Z, Liu X, Peng Z, Wan Y, Ji Y, He W, Wan W, Luo J, Guo H. , AHD2.0: an update version of Arabidopsis Hormone Database for plant systematic studies. , Nucleic Acids Research. , 2011 , 39: D1123–D1129.

10. Li, H., Lin, D., Dhonukshe, P., Nagawa, S., Chen, D., Friml, J., Scheres, B., Guo, H., Yang, Z. , Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. , Cell Res. , 2011 , 21:970-8

10. Li, H., Lin, D., Dhonukshe, P., Nagawa, S., Chen, D., Friml, J., Scheres, B., Guo, H., Yang, Z. , Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. , Cell Res. , 2011 , 21:970-8

11. Zhao, Q. and Guo, H. , Paradigms and paradox in the ethylene signaling pathway and interaction network. , Mol. Plant. , 2011 , 4:626-34

12. Zhong, S., Shi, H., Xi, Y. and Guo, H. , Ethylene is crucial for cotyledon greening and seedling survival during de-etiolation. , Plant Signaling & Behavior. , 2010 , 5: 739-42

13. An F., Zhao Q., Ji Y., Jiang Z., Yu X., Liu Y., Han Y., Zhang C., Zhang S., Ecker J.R., and Guo H. , Ethylene-induced EIN3 and EIN3-Like1 stabilization are mediated by Proteasomal degradation of EBF1/EBF2 that required EIN2 in Arabidopsis. , The Plant Cell. , 2010 , 22:2384-401

14. Jiang, Z. and Guo, H. , A comparative genomic analysis of plant hormone related genes in different species. , J. Genet. Genomics. , 2010 , 37:219-30

15. Zhong, S., Zhao, M., Shi, T., Shi, H., An, F., Zhao, Q. and Guo, H. , EIN3/EIL1 cooperate with PIF1 to prevent photo-oxidation and promote greening of Arabidopsis seedlings. , Proc. Natl. Acad. Sci. USA. , 2009 , 106: 21431-36.

16. Peng, Z., Zhou, X., Li, L., Yu, X., Li, H., Jiang, Z., Cao, G., Bai, M., Wang, X., Jiang, C., Lu, H., Hou, X., Qu, Li.,Wang, Z., Zuo, J., Fu, X., Su, Z., Li, S., and Guo, H. , Arabidopsis Hormone Database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis. , Nucleic Acids Research , 2009 , 37: 975-982.

17. Chen, H., Xue, L., Chintamanani, S., Germain, H., Lin, H., Cui, H., Cai, R., Zuo, J., Tang, X., Li, X., Guo, H., and Zhou, J. , ETHYLENE INSENSITIVE3 and ETHYLENE INSENSITIVE3-LIKE1 repress SALICYLIC ACID INDUCTION DEFICIENT2 expression to negatively regulate plant innate immunity in Arabidopsis. , The Plant Cell , 2009 , 21:2527-40.

18. Li, H., Wong, W., Zhu L., Guo, H., Ecker, J.R., and Li, N. , Phosphoproteomic analysis of ethylene-regulated protein phosphorylation in etiolated seedlings of Arabidopsis mutant ein2 using two-dimensional separations coupled with a hybrid quadruple time-of-flight mass spectrometer. , Proteomics , 2009 , 9:1646-61.

19. Zhu, Z. and Guo, H. , Genetic basis of ethylene perception and signal transduction in Arabidopsis. , Journal of Integrative Plant Biology , 2008 , 50: 808-815.

20. Li, H. and Guo, H. , Molecular basis of the ethylene signaling and response pathway in Arabidopsis. , Journal of Plant Growth Regulation , 2007 , 26: 106-117.

21. Olmedo, G., Guo, H.,* Gregory, B., Nourizadeh, S., Aguilar-Henonin, L., Li, H., An, F., Guzman, P., and Ecker, J.R. , ETHYLENE-INSENSITIVE5 encodes a 5'→3' exoribonuclease required for posttranscriptional regulation of the EIN3-targeting F-box proteins EBF1/2. , Proc. Natl. Acad. Sci. USA. , 2006 , 103: 13286-13293 (* co-first author).

22. An, F., and Guo, H. , Molecular Mechanism of Ethylene Signal Transduction. , Chinese Botany Bulletin , 2006 , 23: 531-542.

23. Guo, H. and Ecker, J.R. , The ethylene signaling pathway: new insights. , Curr Opin Plant Biol. , 2004 , 7: 40-49.

24. Guo, H. and Ecker, J.R. , Plant responses to ethylene gas are mediated by SCFEBF1/EBF2-dependent proteolysis of EIN3 transcription factor. , Cell , 2003 , 115: 667-677.

25. Shalitin, D., Yang, H., Mockler T., Maymon, M., Guo, H., Whitelam, G., and Lin, C. , Regulation of Arabidopsis cryptochrome 2 by blue light-dependent phosphorylation. , Nature , 2002 , 417: 763-767.

26. Guo, H., Mockler, T., Duong, H., and Lin, C. , SUB1, an Arabidopsis calcium-binding protein involved in cryptochrome and phytochrome co-action. , Science , 2001 , 291: 487-490.

27. Guo, H., Duong, H., Ma, N., and Lin, C. , The Arabidopsis blue light receptor cryptochrome 2 is a nuclear protein regulated by a blue light-dependent post-transcriptional mechanism , Plant J. , 1999 , 19: 279-87.

28. Mockler, T., Guo, H., Yang, H., Duong, H., and Lin, C. , Antagonistic actions of the Arabidopsis cryptochromes and phytochrome B in the regulation of floral induction. , Development , 1999 , 126: 2073-2082.

29. Guo, H., Yang, H., Mockler, T., and Lin, C. , Regulation of flowering time by Arabidopsis photoreceptors. , Science , 1998 , 279: 1360-1363.

30. Lin, C., Yang, H., Guo, H., Mockler, T., Chen, T., and Cashmore, A.R. , Enhancement of blue-light sensitivity of Arabidopsis seedling by a blue light receptor cryptochrome 2. , Proc. Natl. Acad. Sci. USA , 1998 , 95: 2686-2690.
 

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