V: Construction and modeling of plant hormones networks
It has been found that many biological processes are coordinately regulated by multiple hormones. We have built an Arabidopsis Hormone Database (AHD: http://ahd.cbi.pku.edu.cn/) by integrating a large body of experimental data and computational annotations. The aim of the Arabidopsis hormone database is to provide a systematic and comprehensive view of morphological phenotypes and regulatory genes participating in numerous plant hormone responses. In the future, we will generate an atlas of plant hormone biology, and make theoretical models to describe, verify and predict the functions and actions of plant hormones.
VI: Hormonal regulation of plant ageing
Plant ageing is quite distinctive and unique compared to animal or human ageing. The proper control of plant ageing programs (such as leaf senescence, flower senescence) has tremendous potential for agricultural good and thus is of great interest to scientists in recent years. The leaf senescence is the final developmental phase of a plant leaf, which is influenced by several phytohormones, with cytokinin and ethylene having the most important roles in delaying or inducing leaf senescence, respectively. Currently we are interested in uncovering the regulatory mechanisms of ethylene and cytokinin on leaf senescence. Also, we have recently built a Leaf Senescence Database (LSD: http://www.eplantsenescence.org/), and attempt to study leaf senescence in a more systematic way.
主要发表文章:
1.Wen X.,Zhang C.,Ji Y.,Zhao Q.,He W.,An F.,Jiang L.,Guo H.(2012).Activation of ethylene signaling is mediated by nuclear translocation of the cleaved EIN2 carboxyl terminus.Cell Res.2012.Oct 16
2.Zhong S.,Shi H.,Xue C.,Wang L.,Xi Y.,Li J.,Quail PH.,Deng XW.,Guo H.(2012).Amolecular framework of light-conrolled phytohormone action in Arabidopsi.Curr Biol.2012 Aug 21;22(16):1530-5.
3.An F., Zhang X., Zhu Z., Ji Y., He W., Jiang Z., Li M., Guo H.(2011). Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings.Cell Res.22(5):915-27.
4.Guo H.(2011). Understanding the mode of phytohormones' action in plants.Sci China Life Sci.54(11):1062-3.
5.Stepanova AN, Yun J., Robles LM., Novak O., He W., Guo H., Ljung K., Alonso JM.(2011). The Arabidopsis YUCCA1 flavin monooxygenase functions in the indole-3-pyruvic acid branch of auxin biosynthesis.Plant Cell.23(11):3961-73.
6. He, W., Brumos J., Li, H., Ji, Y., Ke, M., Gong, X., Zeng, Q., Li, W., Zhang, X., An, F., Wen, X., Li, P., Chu, J., Sun, X., Yan, C., Yan N., Xie, D., Raikhel, N., Yang, Z., Stepanova, A.N., Alonso, J.M. and Guo, H. , A small-molecule screen identifies L-Kynurenine as a competitive inhibitor of TAA1 in ethylene-directed auxin biosynthesis and root growth. , The Plant Cell. , 2011 Nov;23(11):3944-60.
7. Zhu, Z., An, F., Feng, Y., Li, P., Xue, L., A, M., Jiang, Z., Kim, J., To, T., Li, W., Yu, Q., Dong, Z., Chen, W., Seki, M., Zhou, J. and Guo, H. , Release of JAZ- and HDA6-associated repression on EIN3/EIL1 mediates jasmonate and ethylene signaling synergy in Arabidopsis. , Proc. Natl. Acad. Sci. USA. , 2011 , 108:12539-44.
8. Liu X., Li Z. Jiang Z., ZhaoY., Peng J., Jin J., Guo H*., Luo J. , LSD: a leaf senescence database. , Nucleic Acids Research. , 2011 , 39: D1103–D1107. ( *Co-Corresponding Author)
9. Jiang Z, Liu X, Peng Z, Wan Y, Ji Y, He W, Wan W, Luo J, Guo H. , AHD2.0: an update version of Arabidopsis Hormone Database for plant systematic studies. , Nucleic Acids Research. , 2011 , 39: D1123–D1129.
10. Li, H., Lin, D., Dhonukshe, P., Nagawa, S., Chen, D., Friml, J., Scheres, B., Guo, H., Yang, Z. , Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. , Cell Res. , 2011 , 21:970-8
10. Li, H., Lin, D., Dhonukshe, P., Nagawa, S., Chen, D., Friml, J., Scheres, B., Guo, H., Yang, Z. , Phosphorylation switch modulates the interdigitated pattern of PIN1 localization and cell expansion in Arabidopsis leaf epidermis. , Cell Res. , 2011 , 21:970-8
11. Zhao, Q. and Guo, H. , Paradigms and paradox in the ethylene signaling pathway and interaction network. , Mol. Plant. , 2011 , 4:626-34
12. Zhong, S., Shi, H., Xi, Y. and Guo, H. , Ethylene is crucial for cotyledon greening and seedling survival during de-etiolation. , Plant Signaling & Behavior. , 2010 , 5: 739-42
13. An F., Zhao Q., Ji Y., Jiang Z., Yu X., Liu Y., Han Y., Zhang C., Zhang S., Ecker J.R., and Guo H. , Ethylene-induced EIN3 and EIN3-Like1 stabilization are mediated by Proteasomal degradation of EBF1/EBF2 that required EIN2 in Arabidopsis. , The Plant Cell. , 2010 , 22:2384-401
14. Jiang, Z. and Guo, H. , A comparative genomic analysis of plant hormone related genes in different species. , J. Genet. Genomics. , 2010 , 37:219-30
15. Zhong, S., Zhao, M., Shi, T., Shi, H., An, F., Zhao, Q. and Guo, H. , EIN3/EIL1 cooperate with PIF1 to prevent photo-oxidation and promote greening of Arabidopsis seedlings. , Proc. Natl. Acad. Sci. USA. , 2009 , 106: 21431-36.
16. Peng, Z., Zhou, X., Li, L., Yu, X., Li, H., Jiang, Z., Cao, G., Bai, M., Wang, X., Jiang, C., Lu, H., Hou, X., Qu, Li.,Wang, Z., Zuo, J., Fu, X., Su, Z., Li, S., and Guo, H. , Arabidopsis Hormone Database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis. , Nucleic Acids Research , 2009 , 37: 975-982.
17. Chen, H., Xue, L., Chintamanani, S., Germain, H., Lin, H., Cui, H., Cai, R., Zuo, J., Tang, X., Li, X., Guo, H., and Zhou, J. , ETHYLENE INSENSITIVE3 and ETHYLENE INSENSITIVE3-LIKE1 repress SALICYLIC ACID INDUCTION DEFICIENT2 expression to negatively regulate plant innate immunity in Arabidopsis. , The Plant Cell , 2009 , 21:2527-40.
18. Li, H., Wong, W., Zhu L., Guo, H., Ecker, J.R., and Li, N. , Phosphoproteomic analysis of ethylene-regulated protein phosphorylation in etiolated seedlings of Arabidopsis mutant ein2 using two-dimensional separations coupled with a hybrid quadruple time-of-flight mass spectrometer. , Proteomics , 2009 , 9:1646-61.
19. Zhu, Z. and Guo, H. , Genetic basis of ethylene perception and signal transduction in Arabidopsis. , Journal of Integrative Plant Biology , 2008 , 50: 808-815.
20. Li, H. and Guo, H. , Molecular basis of the ethylene signaling and response pathway in Arabidopsis. , Journal of Plant Growth Regulation , 2007 , 26: 106-117.
21. Olmedo, G., Guo, H.,* Gregory, B., Nourizadeh, S., Aguilar-Henonin, L., Li, H., An, F., Guzman, P., and Ecker, J.R. , ETHYLENE-INSENSITIVE5 encodes a 5'→3' exoribonuclease required for posttranscriptional regulation of the EIN3-targeting F-box proteins EBF1/2. , Proc. Natl. Acad. Sci. USA. , 2006 , 103: 13286-13293 (* co-first author).
22. An, F., and Guo, H. , Molecular Mechanism of Ethylene Signal Transduction. , Chinese Botany Bulletin , 2006 , 23: 531-542.
23. Guo, H. and Ecker, J.R. , The ethylene signaling pathway: new insights. , Curr Opin Plant Biol. , 2004 , 7: 40-49.
24. Guo, H. and Ecker, J.R. , Plant responses to ethylene gas are mediated by SCFEBF1/EBF2-dependent proteolysis of EIN3 transcription factor. , Cell , 2003 , 115: 667-677.
25. Shalitin, D., Yang, H., Mockler T., Maymon, M., Guo, H., Whitelam, G., and Lin, C. , Regulation of Arabidopsis cryptochrome 2 by blue light-dependent phosphorylation. , Nature , 2002 , 417: 763-767.
26. Guo, H., Mockler, T., Duong, H., and Lin, C. , SUB1, an Arabidopsis calcium-binding protein involved in cryptochrome and phytochrome co-action. , Science , 2001 , 291: 487-490.
27. Guo, H., Duong, H., Ma, N., and Lin, C. , The Arabidopsis blue light receptor cryptochrome 2 is a nuclear protein regulated by a blue light-dependent post-transcriptional mechanism , Plant J. , 1999 , 19: 279-87.
28. Mockler, T., Guo, H., Yang, H., Duong, H., and Lin, C. , Antagonistic actions of the Arabidopsis cryptochromes and phytochrome B in the regulation of floral induction. , Development , 1999 , 126: 2073-2082.
29. Guo, H., Yang, H., Mockler, T., and Lin, C. , Regulation of flowering time by Arabidopsis photoreceptors. , Science , 1998 , 279: 1360-1363.
30. Lin, C., Yang, H., Guo, H., Mockler, T., Chen, T., and Cashmore, A.R. , Enhancement of blue-light sensitivity of Arabidopsis seedling by a blue light receptor cryptochrome 2. , Proc. Natl. Acad. Sci. USA , 1998 , 95: 2686-2690.