科研领域描述
具有自我更新能力和分化潜能的干细胞是哺乳动物胚胎发育过程中以及成体中的关键种类的细胞，对各种干细胞进行深入研究是理解哺乳动物发育、生长机制的关键，也是将干细胞应用于临床再生医学、治疗人类疾病的前提。本实验室主要围绕哺乳动物早期胚胎发育、研究胚胎干细胞（Embryonic Stem cells）和上胚层干细胞（Epi-Stem cells）的自我更新能力（Self-renewal ability）和多能性（Pluripotency）调控的分子机理，特别是表观遗传学调控机理（Epigenetic regulation），以及相关的原始生殖细胞（Primordial Germ cells）发育过程中的表观遗传学重编程（Epigenetic reprogramming）机理。利用我们发展的单细胞microRNA表达谱分析技术（Single cell microRNA profiling）、基于深度测序的单细胞数字转录组分析技术（single cell RNA-Seq digital transcriptome analysis）、单细胞基因分型技术（Single cell genotyping）、以及染色体免疫共沉淀-深度测序技术（ChIP-Seq）、小鼠胚胎显微操作技术深入分析多能性干细胞的动态基因表达网络和表观遗传学调控。
代表性论文
1. Tang Fuchou, Lao K, Surani MA. , Development and applications of single-cell transcriptome analysis , Nature Methods , 2011 , S6-S11
2. Tang Fuchou, Barbacioru C, Bao S, Lee C, Nordman E, Wang X, Lao K, Surani MA. , Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis , Cell Stem Cell , 2010 , 468 - 478
3. Tang Fuchou , Small RNAs in mammalian germline: tiny for immortal , Differentiation , 2010 , 141-146
4. Tang Fuchou, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA , RNA-Seq analysis to capture the transcriptome landscape of a single cell , Nature Protocols , 2010 , 516-535
5. Bao S*, Tang Fuchou*, Li X, Hayashi K, Gillich A, Lao K, Surani MA , Epigenetic reversion of postimplantation epiblast to pluripotent embryonic stem cells , Nature , 2009 , 1292 - 1295 (*: co-first author)
6. Tang Fuchou*, Barbacioru C*, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch BB, Siddiqui A, Lao K, Surani MA , mRNA-Seq whole transcriptome analysis of a single cell , Nature Methods , 2009 , 377 - 382 (*: co-first author)
7. Hayashi K, Lopes SM, Tang Fuchou, Surani MA , Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states , Cell Stem Cell , 2008 , 391 - 401
8. Tang Fuchou*, Kaneda M*, O'Carroll D, Hajkova P, Barton SC, Sun YA, Lee C, Tarakhovsky A, Lao K, Surani MA , Maternal microRNAs are essential for mouse zygotic development , Genes & Development , 2007 , 644 - 648 (*: co-first author)
9. Tang Fuchou, Hajkova P, Barton SC, O'Carroll D, Lee C, Lao K, Surani MA , 220-plex microRNA expression profile of a single cell , Nature Protocols , 2006 , 1154 - 1159
10. Tang Fuchou, Hajkova P, Barton SC, Lao K, Surani MA , MicroRNA expression profiling of single whole embryonic stem cells , Nucleic Acids Research , 2006 , e9 |
