研究领域：
真核基因定点修饰及高通量功能基因组学，疾病与感染的分子机制。
研究方向：
着重发展真核基因修饰技术（i.e. TALE & CRISPR systems）及高通量功能基因组学，在此基础上通过多种手段研究人源宿主细胞参与病原微生物感染的蛋白、信号通路及分子机制，为发展宿主导向的治疗手段提供新的药物靶点和思路。在生物技术方面关注真核基因定点修饰、功能基因组学以及基因治疗；对疾病与感染的机制研究，特别关注艰难梭菌（Clostridium difficile）毒素侵染机制以及病毒性肝炎（i.e. HCV & HBV）的致病机理和治疗。
Research Interests:
Our laboratory is interested in studying the molecular mechanism of human diseases, especially the host response to microbial pathogenicity. The combination of forward and reverse genetic means is employed, often in a high-throughput fashion, for the identification of host genes of interest. Efforts have also been put into the development and application of eukaryotic gene editing techniques, such as TALENs and CRISPR/Cas9 systems. The current focuses of diseases include Clostridium difficile infections, anthrax toxicity, HCV and HBV.
代表性论文
1. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W , High-throughput Screening of a CRISPR/Cas Library for Functional Genomics in Human Cells , Nature , 2014 , doi: 10.1038/nature13166 , [Full Text] , (AOP)
2. Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, and Wei WS , Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake , Sci China Life Sci , 2014 , 57: 1–13, doi: 10.1007/s11427-014-4646-x , [Full Text] , Cover story
3. Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W , A Complete Decoding of DNA Recognition by TAL Effector RVDs , Cell Res. , 2014 , doi: 10.1038/cr.2014.19. , [Full Text] , [Epub ahead of print]
4. Yang J, Yuan P, Wen D , Sheng Y, Zhu S, Yu Y, Gao X and Wei W , ULtiMATE system for rapid assembly of customized TAL effectors , PLoS One , 2013 , 8:e75649 , [Abstract] , [Full Text]
5. Lih C, Wei W, and Cohen SN. , Txr1: a transcriptional regulator of thrombospondin-1 that modulates cellular sensitivity to taxanes. , Genes Dev. , 2006 , 20, 2082-2095. , [Abstract] , [Full Text]
6. Wei W, Lu Q, Chaudry J, Leppla S, and Cohen SN. , The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. , Cell , 2006 , 124, 1141-1154. , [Abstract] , [Full Text]
7. Lu Q, Wei W, Kowalski P, Chang A, and Cohen SN , EST-based genome-wide gene inactivation identifies ARAP3 as a host protein affecting cellular susceptibility to anthrax toxin , Proc. Natl. Acad. Sci. USA , 2004 , 101, 17246-51 , [Abstract] , [Full Text]
8. Wei W, Plovanich Jones A, Deng W, Jin Q, Collmer A, Huang H, and He SY , The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato , Proc. Natl. Acad. Sci. USA , 2000 , 97, 2247-52 , [Full Text]
9. Roine E, Wei W*, Yuan J, Nurmiaho-Lassila EL, Kalkkinen N, Romantschuk M, He SY , Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000 , Proc. Natl. Acad. Sci. USA , 1997 , 94, 3459-64 , [Full Text] , (*Co-first author)
|
