科研领域描述
利用核磁共振（NMR）技术，结合其它生物化学及分子生物学手段，研究生物大分子结构及其相互作用，以期理解其结构-功能关系，揭示其作用的分子机理。目前主要的研究对象包括细胞凋亡因子Bcl-xL、抑癌基因HREV-107家族蛋白、结核杆菌nucleoid protein Lsr2、SARS病毒主蛋白酶
We are interested in studying 3D structures of proteins and their interactions with other proteins, nucleic acids, and small molecule ligands, using NMR spectroscopy along with other techniques in biochemistry and molecular biology, aiming to reveal protein structure-function relationship and molecular mechanisms for protein functions, including the roles of anti-tumor gene HREV-107 family proteins in cell death, the structural basis of bacterial nucleoid-associated proteins in transcription regulation, the function of pro-apoptotic protein Bcl-XL in autophagy, the mechanism of glutaredoxins in iron and oxygen sensing, and protein folding using SARS-CoV main protease as a model.
代表性论文
1. B. R.G. Gordon, Y Li, A. Coteb, M. T. Weirauch, P. Ding, T. R. Hughes, W. W. Navarre, B. Xia*, and J. Liu* , “Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins” , Proc. Natl. Acad. Sci. , 2011 , 108:10690-5
2. B. Xia* and X. Kang , “Activation and Maturation of SARS-CoV Main Protease” , Protein Cell , 2011 , 2: 282-90
3. F. Song, X. Zhang, X. Ren, P. Zhu, J. Xu, L. Wang, Y. Li, N. Zhong, Q. Ru, D. Zhang, J. Jiang, B. Xia*, & Z. Chen* , “Cyclophilin A (CyPA) induces chemotaxis independent of its peptidylprolyl Cis-trans isomerase activity: Direct binding between CyPA and the ectodomain of CD147” , J. Biol. Chem. , 2011 , 286: 8197-203
4. J. Zhou, J. Lin, C. Zhou, X. Deng, & B. Xia* , “An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein” , Acta Biochim. Biophys. Sin , 2011 , 43: 239-44
5. J. Zhou, J. Lin, C. Zhou, X. Deng, & B. Xia* , “Cytotoxicity of Red Fluorescent Protein DsRed is Associated with the Suppression of Bcl-xL Translation” , FEBS Lett. , 2011 , 585: 821-7
6. L. Wang, X. Ren, Y. Li, N. Rouhier, J. P. Jacquot, C. Jin, B. Xia* , "1H, 13C, and 15N resonance assignments of reduced GrxS14 from Populus tremula x tremuloides". , Biomol NMR Assign , 2011 , 5, 121-4
7. W. Gong, J. Wang, Z. Chen, B. Xia*, and G. Lu* , “Solution Structure of LCI, a Novel Antimicrobial Peptide from Bacillus subtilis” , Biochemistry , 2011 , 50: 3621-7
8. B. R.G. Gordon, Y. Li , L. Wang , A. Sintsova , H. V. Bakel , S. Tian, W. W. Navarre, B. Xia*, and J. Liu* , “Lsr2 is A Nucleoid-Associated Protein that Targets AT-Rich Sequences and Virulence Genes in Mycobacterium tuberculosis”, , Proc. Natl. Acad. Sci. , 2010 , 107: 5154-5159
9. J. Hu, D. Li, X.-D. Su, C. Jin, and B. Xia* , “Solution Structure and Conformational Heterogeneity of Acylphosphatase from Bacillus subtilis” , FEBS Lett. , 2010 , 584: 2852-2856
10. J. Lin, N. Wang, Y. Li, Z. Liu, S. Tian, L. Zhao, Y. Zheng, S. Liu, S. Li, C. Jin, and B. Xia , “LEC-BiFC: A New Method for Rapid Assay of Protein Interaction” , Biotech. Histochem. , 2010 , DOI: 10.3109/10520295.2010.483068
11. S. Tian, J. Lin, J. Zhou, X. Wan, Y. Li, X. Ren, W. Yu, W. Zhong, J. Xiao, F. Sheng, Y. Chen, C. Jin, S. Li, Z. Zheng, and B. Xia* , “Beclin 1-independent autophagy induced by a Bcl-XL/Bcl-2 targeting compound Z18” , Autophagy , 2010 , 6: 1032-41
12. S. Zhang, N. Zhong, F. Xue, X. Kang, X. Ren, J. Chen, C. Jin, Z. Lou and B. Xia* , “3D domain swapping as a mechanism to lock the active conformation in a super-active octamer of SARS-CoV main protease” , Protein Cell , 2010 , 1: 371–83
13. S. Zhang, N. Zhong, X. Ren, C. Jin, B. Xia* , "(1)H, (13)C and (15)N resonance assignments of SARS-CoV main protease N-terminal domain" , Biomol NMR Assign , 2010 , Biomol NMR Assign
14. X. Ren, J. Lin, C. Jin, and B. Xia* , “Solution structure of the N-terminal catalytic domain of human H-REV107 - a novel circular permutated NlpC/P60 domain”, , FEBS Lett. , 2010 , 584:4222-6
15. X. Ren, J. Lin, C. Jin, and B. Xia* , “1H, 13C and 15N resonance assignments of human H-REV107 N-terminal domain” , Biomol. NMR Assign , 2010 , 4: 175-8
16. J. Hu, G. Cui, C. Li, C. Liu, E. Shang, L. Lai, C. Jin, J. Wang, and B. Xia* , “Structure and Novel Functional Mechanism of Drosophila SNF in Sex-lethal Splicing” , PloS ONE , 2009 , 4: e6890
17. J. Lin, Z. Zheng, Y. Li, W. Yu, W. Zhong, S. Tian, F. Zhao, X. Ren, J. Xiao, N. Wang, S. Liu, L. Wang, F. Sheng, Y. Chen, C. Jin, S. Li, and B. Xia* , “A Novel Bcl-XL Inhibitor Z36 that Induces Autophagic Cell Death in Hela Cells” , Autophagy , 2009 , 5: 314-320
18. N. Guo, R. Zhang, F. Song, J. He, B. Xia, and Z. Abliz , “Characterization of Acid-Induced Protein Conformational Changes and Noncovalent Complexes in Solution by Using Coldspray Ionization Mass Spectrometry” , J. Am. Soc. Mass. Spectrom. , 2009 , 20: 845-51
19. N. Zhong, S. Zhang, F. Xue, X. Kang, P. Zou, J. Chen, C. Liang, Z. Rao, C. Jin, Z. Lou, and B. Xia* , “C-Terminal Domain of SARS-CoV Main Protease Can Form a 3D Domain-Swapped Dimer” , Protein Sci. , 2009 , 18: 839-44
20. S. Fan, Y. Feng, Z. Wei, B. Xia*and W. Gong , “Solution Structure of Synbindin Atypical PDZ Domain and Interaction with Syndecan-2” , Protein Pept. Lett. , 2009 , 16: 189-195
21. W. Gong, Y. Li, G. Cui, J. Hu, H. Fang, C. Jin, and B. Xia* , “Solution Structure and Catalytic Mechanism of Human Protein Histidine Phosphatase 1”, , Biochem. J. , 2009 , 418: 337-344
22. W. Yu, J. Hu, B. Yu, W. Xia, C. Jin, and B. Xia* , “Solution Structure of GSP13 From Bacillus subtilis Exhibits an S1 Domain Related to Cold Shock Proteins” , J. Biomol. NMR. , 2009 , 43: 255-259
23. W. Yu, J. Lin, C. Jin, and B. Xia* , “Solution Structure of Human Zeta-COP: Direct Evidences for Structural Similarity between COP I and Clathrin-Adaptor Coats” , J. Mol. Biol. , 2009 , 386: 903-912
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