科研领域描述
一些参与基本生物学过程的重要蛋白质的功能失调会影响细胞的正常生理功能，并可能导致炎症、肿瘤在内的多种疾病，因此，对这些蛋白的结构和功能开展研究具有重要的生物学意义。研究室主要选取一些与炎症、肿瘤等人类重大疾病相关蛋白及一些功能未知的但可能具有重要生物学意义的新型人类蛋白为靶蛋白，研究其结构和功能的分子基础，研究蛋白质与蛋白质以及蛋白质与其它生物分子相互识别和相互作用的分子机制、调控网络关系，以及蛋白质功能的失调与一些重大疾病的发生和发展的关系及分子机理。
本实验室主要兴趣在于：
1. 蛋白质的泛素化、类泛素化、磷酸化等修饰对NF-κB、p53等信号通路以及DNA损伤应答的调节作用及其机制。
2. 炎症调控相关蛋白作用的分子机制及其与癌症等炎症相关疾病的关联性。
3. 流感、乙肝等病毒重要蛋白的结构和功能研究。
代表性论文
1. Hu B, Li SZ, Zhang XD, Zheng XF , HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation , Nucleic Acid Res , 2014 , Accepted
2. Peng YY, Xu RD, Zheng XF , HSCARG negatively regulates the cellular antiviral RIG-I like receptor signaling pathway by inhibiting TRAF3 ubiquitination via recruiting OTUB1 , PLoS Pathogen , 2014 , Accepted
3. Li G, Qu L, Ma S, Wu Y, J CW, Zheng XF , Structures determination of human Fas Apoptosis Inhibitory Molecule and identification of the critical residues linking the interdomain interaction to the anti-apoptotic activity , Acta crystallgrophy D , 2014 , Accepted
4. Li TT, Guan JH, Huang ZJ, Hu X, Zheng XF , RNF168-mediated H2A neddylation antagonizes its ubiquitination and regulates DNA damage repair , Journal Cell Science , 2014 , Accepted
5. Xiao W, Meng G, Zhao Y, Yuan H, Li T, Peng Y, Zhao Y, Luo M, Zhao, WM, Li Z, Zheng XF , Human SI-CLP aggravates the inflammation of the arthritis and is a potential macrophage inflammatory regulator , Arthritis Rheumatol , 2014 , doi: 10.1002/art.38356
6. Zhang JF, Bai DM, Ma XW, Guan JH, Zheng XF , hCINAP is a novel regulator of ribosomal protein-HDM2-p53 pathway by controlling NEDDylation of ribosomal protein S14 , Oncogene , 2014 , 33, 246-254. doi: 10.1038/onc.2012.560 ,
7. Meng G, An X, Ye, S, Liu Y, Zhu W, Zhang R, Zheng XF , The crystal structure of LidA, a translocated substrate of the Legionella pneumophila Type IV secretion system, reveals a novel folding , Protein & Cell , 2013 , 4(12):897-900
8. Bai DM, Zhang J, Xiao WC, Zheng XF , Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress , Nucleic Acid Res , 2013 , doi: 10.1093/nar/gkt971
9. Bai XY, Zheng XF , Tip-to-tip interaction in the crystal packing of PACSIN 2 is important in regulating tubulation activity , Protein & Cell , 2013 , 4(3): 211–219
10. Liu Y, Qin K, Meng G, Zhang J, Zhou JF, Zhao GY, Luo M, Zheng XF , Structural and Functional Characterization of K339T Substitution Identified in the PB2 Cap-binding Pocket of Influenza A Virus , J Biol. Chem , 2013 , 288(16): 11013-11023
11. Xiao WC, Peng YY, Li Z, Zheng XF , HSCARG inhibits NADPH oxidase activity through regulation of the expression of p47phox. , PLoS ONE , 2013 , 8(3): e59301
12. Feng X, Yang R, Zheng XF*, Zhang F , Identification of a novel nuclear-localized adenylate kinase 6 from Arabidopsis thaliana as an essential stem growth factor. , Plant Physiol Biochem. , 2012 , 61: 180-186
13. Zhang M, Hu B, Li T, Peng Y, Guan J, Lai S, Zheng XF , A CRM1-dependent nuclear export signal controls nucleocytoplasmic translocation of HSCARG, which regulates NF-κB activity. , Traffic , 2012 , 13(6): 790-799 ,
14. Bai X, Meng G, Luo M, Zheng XF , The Rigidity of the Wedge Loop in PACSIN 3 is a Key Factor in Dictating the Diameters of Tubules , J Biol. Chem. , 2012 , In press
15. Yu Q, Zheng XF , The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect important for regulating pyrophosphorylase activity. , Biochem J , 2012 , 442(2): 283-291.
16. Meng G, Zhao YM, Todd J Green, Luo M, Zheng XF , The structure of Human SI-CLP reveals a saccharide binding cleft with lower selectivity of sugar binding , J Biol. Chem. , 2010 , 285, 39898-39904 , [Abstract]
17. Zhang JF, Zhang FY, Zheng XF , Deficiency of hCINAP causes defects in Cajal body formation,histone transcription, and cell viability , Cellular and Molecular Life Sciences. , 2010 , 67:1907–1918 ,
18. Gan QN, Li TT, Hu B, Lian M, Zheng XF , HSCARG inhibits activation of NF-κB by interacting with IκB kinase-β , J Cell Sci. , 2009 , 4081-4088 ,
19. Dai XY, Li YY, Meng G, Yao S, Zhao YM, Yu Q, Zhang JF, Luo M and Zheng XF , NADPH is an allosteric regulator of HSCARG. , J Mol. Biol. , 2009 , 387, 1277–1285. ,
20. Heinicke LA, Wong J, Lary J, Nallagatla SR, Diegelman-Parente A, Zheng XF, Cole JL, Philip C Bevilacqua , RNA dimerization promotes PKR dimerization and activation , J Mol Biol. , 2009 , 390(2): 319-338.
21. Li GM, Tang ZT, Meng G, Dai KD, Zhao JD, Zheng XF , Crystallization and preliminary X-ray crystallographic studies of O-methyltransferase from the Anabaena Pcc 7120 , Acta crystallgrophy F , 2009 , In press
22. Lian Min, Zheng Xiaofeng , HSCARG regulates NF-κB activation by promoting the ubiquitination of RelA or COMMD1 in response to changes of cellular NADPH/NADP+ , J Biol. Chem. , 2009 , 284(27): 17998-18006. ,
23. Meng G, Bai X, Green TJ, Luo M, Zheng XF , Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain–containing protein , Protein & Peptide Letter , 2009 , 16(3): 336-338
24. Zhang Y, Lu S, Zhao S, Zheng XF, Long M, Wei L. , Positive selection for the male functionality of a co-retroposed gene in the hominoids , BMC Evol Biol. , 2009 , 9: 252.
25. Zhao YM, Zhang JF, Li HY, Li YY, Ren J, Luo M, Zheng XF , An NADPH sensor protein (HSCARG) downregulates nitric oxide synthesis by association with argininosuccinate synthetase and is essential for epithelial cell viability. , J Biol. Chem. , 2008 , 283(16):11004-11013. , [Abstract] , [Full Text] , This paper has been selected by Science Signaling as Editor's Choice (Citation: N. R. Gough, NADPH Sensor Regulates NO Production. Sci. Signal. 1, ec142 (2008).)
26. Meng G, Zhai RT, Liu B, Zheng XF , Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster. , Biochemistry (Moscow). , 2008 , 73: 38-43.
27. Chen B, Lian M, Luo M, Zheng XF , A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell. , J Biotechnology , 2008 , 137: 8-13
28. Lian M, Zhou X, Chen B, Li C, Gu X, Luo M, Zheng XF , Identification of the critical regions in HBV preS required for its stability. , J Peptide Science. , 2008 , 14(3): 307-312. ,
29. Nallagatla SR, Hwang J, Toroney R, Zheng XF, Cameron CE, Bevilacqua PC , 5'-triphosphate-dependent activation of PKR by RNAs with short stem-loops. , Science , 2007 , 318: 1455-1458. , [Abstract] ,
30. Zheng XF, Dai XY, Zhao YM, Chen Q, Lu F, Yao DQ, Liu XP, Zhang CM, Gu X, Luo M , Restructuring of the dinucleotide binding fold in a NADP(H) sensor protein. , Proc.Natl.Acad.Sci.USA. , 2007 , 104(21): 8809-8814. ,
31. Chen Q, You DL, Liang YH, Su XD, Gu X, Luo M, Zheng XF , Crystal structure of the Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyltransferase L160I mutant: insights into the inhibitor design. , FEBS J. , 2007 , 274: 4408-4415. ,
32. Li CY, Yu Q, Ye ZQ, Sun Y, He QY, Li XM, Zhang WX, Luo JC, Gu X, Zheng XF, Wei LP , A Nonsynonymous SNP in Human Cytosolic Sialidase in a Small Asian Population Results in Reduced Enzyme Activity: Potential Link with Severe Adverse Reactions to Oseltamivir. , Cell Research. , 2007 , 17(4):357-362. ,
33. Lian M, Zhou X, Wei L, Qiu SH, Zhou T, Li L, Gu X, Luo M, Zheng XF , 3. Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients , Virology J. , 2007 , 4: 93. ,
34. Chen Q, Hui R, Sun C, Gu X, Luo M, Zheng XF , Soluble expression, purification, and stabilization of a pro-apoptotic human protein, CARP. , Protein Expression & Purification. , 2006 , 45(2):329-334.
35. Dai X, Gu X, Luo M, Zheng XF , Protein expression, crystallization and preliminary X-ray crystallographic studies on HSCARG from Homo sapiens. , Protein & Peptide Letter. , 2006 , 13(9):955-957.
36. Zhai RT, Meng G, Zhao YM, Liu B, Zhang GF, Zheng XF , A novel nuclear-localized protein with special adenylate kinase properties from Caenorhabditis elegans. , FEBS Letters. , 2006 , 580: 3811-3817.
37. Chen Q, Liang YH, Su XD, Gu X, Zheng XF, Luo M , Novel IMP Binding in Feedback Inhibition of Hypoxanthine-Guanine Phosphoribosyltransferase from Thermoanaerobacter tengcongensis. , J. Mol. Biol. , 2005 , 348(5):1199-1210.
38. Dai X, Chen Q, Lian M, Zhou Y, Zhou M, Lu SY, Chen YJ, Luo JC, Gu X, Jiang Y, Luo M, Zheng XF , Systematic high-yield production of human secreted proteins in Escherichia coli. , Biochem Biophys Research Commun. , 2005 , 332: 593-601.
39. Li D, Liang YH, Zheng XF, Li L, Su XD , Protein preparation, Crystallization and Preliminary X-ray Analysis of YjcG protein from Bacillus subtilis. , Acta Cryst. Sect. F , 2005 , Volume 61（5）Epub
40. Ren H, Wang LY, Bennett M, Liang YH, Zheng XF, Lu F, Li LF, Luo M, Eriksson S,. Zhang CM, Su XD , The Crystal Structure of human AK6: An Adenylate Kinase Isoform Localized to the Cell Nucleus. , Proc.Natl.Acad.Sci.USA. , 2005 , 102 (2):303-8.
41. Ren H, LiangYH, Li R, Ding H, Qiu S, Lu S, An J, Li L, Luo M, Zheng XF, Su XD , Protein preparation, Crystallization and Preliminary X-ray Analysis of human adrenal gland protein AD-004. , Acta Cryst D , 2004 , 60: 1292-1294.
42. Zheng XF and Bevilacqua PC , A novel class of activators of PKR: small double-stranded RNAs with single-stranded tails. , RNA , 2004 , 10:1934-1945.
43. Gong W, Liu H, Niu L, Shi Y, Tang Y, Teng M, Wu J, Liang D, Wang D, Wang J, Ding J, Hu H, Huang Q, Zhang Q, Lu S, An J, Liang Y, Zheng X, Gu X, Su XD , Structural genomics efforts at the Chinese Academy of Sciences and Peking University. , J Struct Funct Genomics. , 2003 , 4(23): 137-139.
44. Zheng XF and Bevilacqua PC. , Efficient construction of long DNA duplexes with internal non-Watson-Crick motifs and modifications. , Nucleic. Acids Res. , 2001 , 29(2) e6.
45. Zheng XF and Bevilacqua PC. , Straightening of bulged RNA by the double-stranded RNA-binding domain from the protein kinase PKR. , Proc.Natl.Acad.Sci.USA , 2000 , 97(26):14162-14167.
46. Zheng XF, Kobayashi Y, Takeuchi M. , Construction of a low serine-type carboxypeptidase producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion. , Appl. Microbiol. Biotechnology , 1998 , 49:39-44.
47. Zheng XF and Huang BQ , Intranuclear and cytoplasmic inclusions in embryogenic and embryoidal cells of Panax quinquefolius. , Acta Bontanica Sinica , 1994 , 35(6): 422-428.
48. Zheng XF and Huang BQ , Marker proteins for somatic embryogenesis in several plants. , Acta Bontanica Sinica , 1994 , 36(3):175-180.
49. Wang L, Zheng XF, Zhang G , Rapid propagation of Leymus giganteus L. , Plant Physiology Communications , 1990 , 3:47-48.
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