本实验室主要兴趣在于：
实验室主要兴趣是斑马鱼内部器官的形成，尤其是心血管系统的发育，并以此为基础，研究人类先天性心脏病的发病机理。 斑马鱼是研究脊椎动物发育和人类疾病的理想模式生物。随着斑马鱼基因组计划的接近完成，我们面临着研究将近30,000个在斑马鱼和其他脊椎动物都保守的基因的功能及其调控的艰巨任务。正向遗传学的诱变技术、反向遗传学的TALEN技术、CRISPR/Cas9技术以及基于TALEN的同源重组技术都已在斑马鱼中得到成功地应用，可以随心所欲地将斑马鱼的任何基因修饰为任何形式，为研究基因功能提供了完美的利器。
Research Description:
The zebrafish is an excellent model animal to study vertebrate development and human diseases. With the near completion of its genome sequence, we face an enormous and immediate challenge to reveal the function and regulation of approximately 30,000 highly conserved genes contained in zebrafish and other vertebrate genomes. Recently, the techniques of TALEN, CRISPR/Cas9 and DNA double strands-break mediated homologous recombination have been developed in zebrafish succefully. Based on these powerful techniques, people can modify any zebrafish gene as they wish. Now we are exploring the cardiac development of zebrafish and the molecular mechanism of the pathogenesis of human congenital heart disease (CHD) by using these techniques.
代表性论文
1. Tong X*#, Zu Y*, Li Z*, Li W, Ying L, Yang J, Wang X, He S, Liu D, Zhu Z, Chen J, Lin S, Zhang B. , Kctd10 regulates heart morphogenesis by repressing the transcriptional activity of Tbx5a in zebrafish. , Nat. Commun. , 2014 , 5:3153. doi: 10.1038/ncomms4153. , *共同第一作者；#通讯作者
2. Xia Z, Tong X, Liang F, Zhang Y, Kuok C, Zhang Y, Liu X, Zhu Z, Lin S, Zhang B. , Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish. , Dev Biol. , 2013 , 381(1):83-96
3. Zu Y*, Tong X*, Wang Z, Liu D, Pan R, Li Z, Hu Y, Luo Z, Huang P, Wu Q, Zhu Z, Zhang B, Lin S. , TALEN mediated precise genome modification by homologous recombination in zebrafish. , Nat. Methods , 2013 , 10(4):329-31 , *共同第一作者 （封面文章并配发评论）
4. Tong X*, Xia Z*, Zu Y, Telfer H, Hu J, Yu J, Liu H, Zhang Q, Sodmergen, Lin S, Zhang B. , ngs (Notochord Granular Surface) Gene Encodes a Novel Type of Intermediate Filament Family Protein Essential for Notochord Maintenance in Zebrafish. , J Biol Chem. , 2013 , 288(4):2711-20
5. Tong X, O'Kelly J, Xie D, Mori A, Lemp N, McKenna R, Miller CW, Koeffler HP , Cyr61 suppresses the growth of non-small-cell lung cancer cells via the beta-catenin-c-myc-p53 pathway , Oncogene , 2004 , 23(28):4847-55
6. Xie D, Yin D, Tong X, O'Kelly J, Mori A, Miller C, Black K, Gui D, Said JW, Koeffler HP , Cyr61 is overexpressed in gliomas and involved in integrin-linked kinase-mediated Akt and beta-catenin-TCF/Lef signaling pathways , Cancer Res. , 2004 , 64(6):1987-96
7. Tong X, Xie D, Roth W, Reed J, Koeffler HP , NADE (p75NTR-associated cell death executor) suppresses cellular growth in vivo , Int J Oncol. , 2003 , 22(6): 1357-62
8. Hoffman WK, Tong XJ, Ajioka RS, Kushner JP, Koeffler HP , Mutation analysis of transferrin-receptor 2 in patients with atypical hemochromatosis , Blood , 2002 , 100 (3): 1099-100
9. Tong X, Kawabata H, Koeffler HP. , Iron deficiency can upregulate expression of transferrin receptor at both the mRNA and protein level , Br J Haematol. , 2002 , 116(2):458-64
10. Tong X, Xie D, O'Kelly J, Miller CW, Muller-Tidow C, Koeffler HP. , Cyr61, a member of CCN family, is a tumor suppressor in non-small cell lung cancer. , J Biol Chem. , 2001 , 276(50):47709-14
11. Kawabata H, Germain RS, Ikezoe T, Tong X, Green EM, Gombart AF, Koeffler HP , Regulation of expression of murine transferrin receptor 2 , Blood , 2001 , 98(6):1949-54
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